| 1 | Sat 16 Jan 2021 | 140-2 v Swansea CityBarnsley 0-2 Swansea City | Sky Bet Championship |
| 2 | Tue 19 Jan 2021 | 140-1 v WatfordWatford 1-0 Barnsley | Sky Bet Championship |
| 3 | Wed 27 Jan 2021 | 142-2 v Cardiff CityBarnsley 2-2 Cardiff City | Sky Bet Championship |
| 4 | Sat 30 Jan 2021 | 140-0 v Nottingham ForestNottingham Forest 0-0 Barnsley | Sky Bet Championship |
| 5 | Sun 14 Feb 2021 | 142-0 v BrentfordBrentford 0-2 Barnsley | Sky Bet Championship |
| 6 | Wed 17 Feb 2021 | 142-1 v Blackburn RoversBarnsley 2-1 Blackburn Rovers | Sky Bet Championship |
| 7 | Sat 20 Feb 2021 | 141-0 v Bristol CityBristol City 0-1 Barnsley | Sky Bet Championship |
| 8 | Wed 24 Feb 2021 | 142-0 v Stoke CityBarnsley 2-0 Stoke City | Sky Bet Championship |
| 9 | Sat 27 Feb 2021 | 142-1 v MillwallBarnsley 2-1 Millwall | Sky Bet Championship |
| 10 | Wed 3 Mar 2021 | 143-1 v Queens Park RangersQueens Park Rangers 1-3 Barnsley | Sky Bet Championship |
| 11 | Sat 6 Mar 2021 | 141-0 v Birmingham CityBarnsley 1-0 Birmingham City | Sky Bet Championship |
| 12 | Wed 10 Mar 2021 | 140-0 v Derby CountyBarnsley 0-0 Derby County | Sky Bet Championship |
| 13 | Sat 13 Mar 2021 | 14 3-2 v AFC BournemouthAFC Bournemouth 2-3 Barnsley | Sky Bet Championship |
| 14 | Wed 17 Mar 2021 | 14 3-1 v Wycombe WanderersWycombe Wanderers 1-3 Barnsley | Sky Bet Championship |
| 15 | Sat 20 Mar 2021 | 141-2 v Sheffield WednesdayBarnsley 1-2 Sheffield Wednesday | Sky Bet Championship |
| 16 | Fri 2 Apr 2021 | 141-1 v ReadingBarnsley 1-1 Reading | Sky Bet Championship |
| 17 | Mon 5 Apr 2021 | 142-1 v Luton TownLuton Town 1-2 Barnsley | Sky Bet Championship |
| 18 | Sat 10 Apr 2021 | 142-0 v MiddlesbroughBarnsley 2-0 Middlesbrough | Sky Bet Championship |
| 19 | Sun 18 Apr 2021 | 140-2 v Coventry CityCoventry City 2-0 Barnsley | Sky Bet Championship |
| 20 | Wed 21 Apr 2021 | 14 1-0 v Huddersfield TownHuddersfield Town 0-1 Barnsley | Sky Bet Championship |
| 21 | Sat 24 Apr 2021 | 141-0 v Rotherham UnitedBarnsley 1-0 Rotherham United | Sky Bet Championship |
| 22 | Sat 1 May 2021 | 140-2 v Preston North EndPreston North End 2-0 Barnsley | Sky Bet Championship |
| 23 | Sat 8 May 2021 | 142-2 v Norwich CityBarnsley 2-2 Norwich City | Sky Bet Championship |
| 24 | Mon 17 May 2021 | 140-1 v Swansea CityBarnsley 0-1 Swansea City | Sky Bet Championship Play-off |
| 25 | Sat 22 May 2021 | 141-1 v Swansea CitySwansea City 1-1 Barnsley | Sky Bet Championship Play-off |
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