| 1 | Sun 20 Sep 2020 | 111-1 v FeyenoordFeyenoord 1-1 FC Twente |
| 2 | Fri 25 Sep 2020 | 113-1 v FC GroningenFC Twente 3-1 FC Groningen |
| 3 | Sat 3 Oct 2020 | 111-1 v FC EmmenFC Twente 1-1 FC Emmen |
| 4 | Sat 17 Oct 2020 | 113-0 v Willem IIWillem II 0-3 FC Twente |
| 5 | Sat 24 Oct 2020 | 111-2 v FC UtrechtFC Utrecht 2-1 FC Twente |
| 6 | Sat 31 Oct 2020 | 115-1 v PEC ZwolleFC Twente 5-1 PEC Zwolle |
| 7 | Sat 7 Nov 2020 | 114-2 v ADO Den HaagADO Den Haag 2-4 FC Twente |
| 8 | Sat 5 Dec 2020 | 112-1 v AjaxAjax 1-2 FC Twente |
| 9 | Sun 13 Dec 2020 | 111-3 v AZ AlkmaarFC Twente 1-3 AZ Alkmaar |
| 10 | Sat 19 Dec 2020 | 112-1 v VVV VenloVVV Venlo 1-2 FC Twente |
| 11 | Tue 22 Dec 2020 | 110-2 v Sparta RotterdamFC Twente 0-2 Sparta Rotterdam |
| 12 | Sat 9 Jan 2021 | 114-1 v FC EmmenFC Emmen 1-4 FC Twente |
| 13 | Thu 14 Jan 2021 | 111-3 v AjaxFC Twente 1-3 Ajax |
| 14 | Sun 17 Jan 2021 | 112-2 v FC GroningenFC Groningen 2-2 FC Twente |
| 15 | Sun 24 Jan 2021 | 110-1 v VVV VenloFC Twente 0-1 VVV Venlo |
| 16 | Thu 28 Jan 2021 | 110-0 v Sparta RotterdamSparta Rotterdam 0-0 FC Twente |
| 17 | Sun 31 Jan 2021 | 11 0-0 v sc HeerenveenFC Twente 0-0 sc Heerenveen |
| 18 | Sat 6 Feb 2021 | 110-3 v PSV EindhovenPSV Eindhoven 3-0 FC Twente |
| 19 | Sun 14 Feb 2021 | 112-0 v VitesseVitesse 0-2 FC Twente |
| 20 | Sun 21 Feb 2021 | 112-2 v FeyenoordFC Twente 2-2 Feyenoord |
| 21 | Sat 27 Feb 2021 | 112-2 v Heracles AlmeloHeracles Almelo 2-2 FC Twente |
| 22 | Sat 6 Mar 2021 | 111-1 v Willem IIFC Twente 1-1 Willem II |
| 23 | Sat 13 Mar 2021 | 111-4 v AZ AlkmaarAZ Alkmaar 4-1 FC Twente |
| 24 | Fri 19 Mar 2021 | 110-0 v sc Heerenveensc Heerenveen 0-0 FC Twente |
| 25 | Sat 3 Apr 2021 | 111-2 v VitesseFC Twente 1-2 Vitesse |
| 26 | Sat 10 Apr 2021 | 110-1 v PEC ZwollePEC Zwolle 1-0 FC Twente |
| 27 | Sun 25 Apr 2021 | 111-2 v FC UtrechtFC Twente 1-2 FC Utrecht |
| 28 | Sat 1 May 2021 | 110-3 v Fortuna SittardFortuna Sittard 3-0 FC Twente |
| 29 | Sat 8 May 2021 | 111-1 v Heracles AlmeloFC Twente 1-1 Heracles Almelo |
| 30 | Thu 13 May 2021 | 111-2 v RKC WaalwijkRKC Waalwijk 2-1 FC Twente |
| 31 | Sun 16 May 2021 | 11 3-2 v ADO Den HaagFC Twente 3-2 ADO Den Haag |
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