| 1 | Sun 20 Sep 2020 | 371-1 v FeyenoordFeyenoord 1-1 FC Twente |
| 2 | Sat 3 Oct 2020 | 371-1 v FC EmmenFC Twente 1-1 FC Emmen |
| 3 | Sat 7 Nov 2020 | 374-2 v ADO Den HaagADO Den Haag 2-4 FC Twente |
| 4 | Sun 22 Nov 2020 | 371-1 v PSV EindhovenFC Twente 1-1 PSV Eindhoven |
| 5 | Sat 5 Dec 2020 | 372-1 v AjaxAjax 1-2 FC Twente |
| 6 | Sat 9 Jan 2021 | 374-1 v FC EmmenFC Emmen 1-4 FC Twente |
| 7 | Thu 14 Jan 2021 | 371-3 v AjaxFC Twente 1-3 Ajax |
| 8 | Sun 17 Jan 2021 | 37 2-2 v FC GroningenFC Groningen 2-2 FC Twente |
| 9 | Sun 24 Jan 2021 | 370-1 v VVV VenloFC Twente 0-1 VVV Venlo |
| 10 | Thu 28 Jan 2021 | 370-0 v Sparta RotterdamSparta Rotterdam 0-0 FC Twente |
| 11 | Sat 6 Feb 2021 | 370-3 v PSV EindhovenPSV Eindhoven 3-0 FC Twente |
| 12 | Sun 14 Feb 2021 | 372-0 v VitesseVitesse 0-2 FC Twente |
| 13 | Sun 21 Feb 2021 | 372-2 v FeyenoordFC Twente 2-2 Feyenoord |
| 14 | Sat 6 Mar 2021 | 371-1 v Willem IIFC Twente 1-1 Willem II |
| 15 | Sat 3 Apr 2021 | 371-2 v VitesseFC Twente 1-2 Vitesse |
| 16 | Sat 10 Apr 2021 | 370-1 v PEC ZwollePEC Zwolle 1-0 FC Twente |
| 17 | Sat 1 May 2021 | 370-3 v Fortuna SittardFortuna Sittard 3-0 FC Twente |
| 18 | Sat 8 May 2021 | 371-1 v Heracles AlmeloFC Twente 1-1 Heracles Almelo |
| 19 | Thu 13 May 2021 | 371-2 v RKC WaalwijkRKC Waalwijk 2-1 FC Twente |
| 20 | Sun 16 May 2021 | 373-2 v ADO Den HaagFC Twente 3-2 ADO Den Haag |
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