| 1 | Sat 19 Sep 2020 | 4 1-0 v TorinoFiorentina 1-0 Torino |
| 2 | Sat 26 Sep 2020 | 43-4 v Inter MilanInter Milan 4-3 Fiorentina |
| 3 | Fri 2 Oct 2020 | 41-2 v SampdoriaFiorentina 1-2 Sampdoria |
| 4 | Sun 18 Oct 2020 | 42-2 v SpeziaSpezia 2-2 Fiorentina |
| 5 | Sun 25 Oct 2020 | 43-2 v UdineseFiorentina 3-2 Udinese |
| 6 | Sun 1 Nov 2020 | 40-2 v RomaRoma 2-0 Fiorentina |
| 7 | Sat 7 Nov 2020 | 4 0-0 v ParmaParma 0-0 Fiorentina |
| 8 | Sun 22 Nov 2020 | 40-1 v BeneventoFiorentina 0-1 Benevento |
| 9 | Sun 29 Nov 2020 | 40-2 v AC MilanAC Milan 2-0 Fiorentina |
| 10 | Mon 7 Dec 2020 | 41-1 v GenoaFiorentina 1-1 Genoa |
| 11 | Sun 13 Dec 2020 | 40-3 v AtalantaAtalanta 3-0 Fiorentina |
| 12 | Wed 16 Dec 2020 | 4 1-1 v SassuoloFiorentina 1-1 Sassuolo |
| 13 | Sat 19 Dec 2020 | 41-1 v Hellas VeronaFiorentina 1-1 Hellas Verona |
| 14 | Tue 22 Dec 2020 | 43-0 v JuventusJuventus 0-3 Fiorentina |
| 15 | Sun 3 Jan 2021 | 40-0 v BolognaFiorentina 0-0 Bologna |
| 16 | Sun 10 Jan 2021 | 41-0 v CagliariFiorentina 1-0 Cagliari |
| 17 | Sun 17 Jan 2021 | 40-6 v NapoliNapoli 6-0 Fiorentina |
| 18 | Sat 23 Jan 2021 | 42-1 v CrotoneFiorentina 2-1 Crotone |
| 19 | Fri 29 Jan 2021 | 4 1-1 v TorinoTorino 1-1 Fiorentina |
| 20 | Sun 14 Feb 2021 | 41-2 v SampdoriaSampdoria 2-1 Fiorentina |
| 21 | Fri 19 Feb 2021 | 43-0 v SpeziaFiorentina 3-0 Spezia |
| 22 | Sun 28 Feb 2021 | 40-1 v UdineseUdinese 1-0 Fiorentina |
| 23 | Wed 3 Mar 2021 | 41-2 v RomaFiorentina 1-2 Roma |
| 24 | Sun 7 Mar 2021 | 43-3 v ParmaFiorentina 3-3 Parma |
| 25 | Sat 13 Mar 2021 | 44-1 v BeneventoBenevento 1-4 Fiorentina |
| 26 | Sun 21 Mar 2021 | 42-3 v AC MilanFiorentina 2-3 AC Milan |
| 27 | Sat 3 Apr 2021 | 41-1 v GenoaGenoa 1-1 Fiorentina |
| 28 | Sun 11 Apr 2021 | 4 2-3 v AtalantaFiorentina 2-3 Atalanta |
| 29 | Sat 17 Apr 2021 | 4 1-3 v SassuoloSassuolo 3-1 Fiorentina |
| 30 | Sun 25 Apr 2021 | 41-1 v JuventusFiorentina 1-1 Juventus |
| 31 | Sun 2 May 2021 | 4 3-3 v BolognaBologna 3-3 Fiorentina |
| 32 | Sat 8 May 2021 | 42-0 v LazioFiorentina 2-0 Lazio |
| 33 | Wed 12 May 2021 | 40-0 v CagliariCagliari 0-0 Fiorentina |
| 34 | Sun 16 May 2021 | 4 0-2 v NapoliFiorentina 0-2 Napoli |
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