| 1 | Sat 5 Sep 2020 | 250-1 v Coventry CityMilton Keynes Dons 0-1 Coventry City | Carabao Cup First Round South |
| 2 | Sat 19 Sep 2020 | 25 1-2 v Lincoln CityMilton Keynes Dons 1-2 Lincoln City | Sky Bet League One |
| 3 | Sat 26 Sep 2020 | 250-2 v Crewe AlexandraCrewe Alexandra 2-0 Milton Keynes Dons | Sky Bet League One |
| 4 | Sat 3 Oct 2020 | 251-1 v Ipswich TownMilton Keynes Dons 1-1 Ipswich Town | Sky Bet League One |
| 5 | Tue 6 Oct 2020 | 253-2 v StevenageStevenage 2-3 Milton Keynes Dons | Papa John’s Trophy Group 9 |
| 6 | Wed 21 Oct 2020 | 72-2 v Stoke CityStoke City 2-2 Barnsley | Sky Bet Championship |
| 7 | Sat 24 Oct 2020 | 71-1 v MillwallMillwall 1-1 Barnsley | Sky Bet Championship |
| 8 | Tue 27 Oct 2020 | 73-0 v Queens Park RangersBarnsley 3-0 Queens Park Rangers | Sky Bet Championship |
| 9 | Sat 31 Oct 2020 | 71-0 v WatfordBarnsley 1-0 Watford | Sky Bet Championship |
| 10 | Sat 7 Nov 2020 | 72-0 v Derby CountyDerby County 0-2 Barnsley | Sky Bet Championship |
| 11 | Sat 21 Nov 2020 | 72-0 v Nottingham ForestBarnsley 2-0 Nottingham Forest | Sky Bet Championship |
| 12 | Tue 24 Nov 2020 | 70-1 v BrentfordBarnsley 0-1 Brentford | Sky Bet Championship |
| 13 | Sat 28 Nov 2020 | 71-2 v Blackburn RoversBlackburn Rovers 2-1 Barnsley | Sky Bet Championship |
| 14 | Tue 1 Dec 2020 | 72-1 v Birmingham CityBirmingham City 1-2 Barnsley | Sky Bet Championship |
| 15 | Fri 4 Dec 2020 | 70-4 v AFC BournemouthBarnsley 0-4 AFC Bournemouth | Sky Bet Championship |
| 16 | Wed 9 Dec 2020 | 72-1 v Wycombe WanderersBarnsley 2-1 Wycombe Wanderers | Sky Bet Championship |
| 17 | Sat 12 Dec 2020 | 72-1 v Sheffield WednesdaySheffield Wednesday 1-2 Barnsley | Sky Bet Championship |
| 18 | Tue 15 Dec 2020 | 72-1 v Preston North EndBarnsley 2-1 Preston North End | Sky Bet Championship |
| 19 | Sat 19 Dec 2020 | 70-2 v Swansea CitySwansea City 2-0 Barnsley | Sky Bet Championship |
| 20 | Sat 26 Dec 2020 | 72-1 v Huddersfield TownBarnsley 2-1 Huddersfield Town | Sky Bet Championship |
| 21 | Tue 29 Dec 2020 | 72-1 v Rotherham UnitedRotherham United 1-2 Barnsley | Sky Bet Championship |
| 22 | Sat 2 Jan 2021 | 70-1 v Norwich CityNorwich City 1-0 Barnsley | Sky Bet Championship |
| 23 | Sun 10 Jan 2021 | 72-0 v Tranmere RoversBarnsley 2-0 Tranmere Rovers | Emirates FA Cup Third Round |
| 24 | Sat 16 Jan 2021 | 70-2 v Swansea CityBarnsley 0-2 Swansea City | Sky Bet Championship |
| 25 | Tue 19 Jan 2021 | 70-1 v WatfordWatford 1-0 Barnsley | Sky Bet Championship |
| 26 | Sat 23 Jan 2021 | 71-0 v Norwich CityBarnsley 1-0 Norwich City | Emirates FA Cup Fourth Round |
| 27 | Wed 27 Jan 2021 | 72-2 v Cardiff CityBarnsley 2-2 Cardiff City | Sky Bet Championship |
| 28 | Sat 30 Jan 2021 | 70-0 v Nottingham ForestNottingham Forest 0-0 Barnsley | Sky Bet Championship |
| 29 | Thu 11 Feb 2021 | 70-1 v ChelseaBarnsley 0-1 Chelsea | Emirates FA Cup Fifth Round |
| 30 | Sun 14 Feb 2021 | 72-0 v BrentfordBrentford 0-2 Barnsley | Sky Bet Championship |
| 31 | Wed 17 Feb 2021 | 72-1 v Blackburn RoversBarnsley 2-1 Blackburn Rovers | Sky Bet Championship |
| 32 | Sat 20 Feb 2021 | 71-0 v Bristol CityBristol City 0-1 Barnsley | Sky Bet Championship |
| 33 | Wed 24 Feb 2021 | 72-0 v Stoke CityBarnsley 2-0 Stoke City | Sky Bet Championship |
| 34 | Sat 27 Feb 2021 | 72-1 v MillwallBarnsley 2-1 Millwall | Sky Bet Championship |
| 35 | Wed 3 Mar 2021 | 73-1 v Queens Park RangersQueens Park Rangers 1-3 Barnsley | Sky Bet Championship |
| 36 | Sat 6 Mar 2021 | 71-0 v Birmingham CityBarnsley 1-0 Birmingham City | Sky Bet Championship |
| 37 | Wed 10 Mar 2021 | 70-0 v Derby CountyBarnsley 0-0 Derby County | Sky Bet Championship |
| 38 | Sat 13 Mar 2021 | 73-2 v AFC BournemouthAFC Bournemouth 2-3 Barnsley | Sky Bet Championship |
| 39 | Wed 17 Mar 2021 | 73-1 v Wycombe WanderersWycombe Wanderers 1-3 Barnsley | Sky Bet Championship |
| 40 | Sat 20 Mar 2021 | 71-2 v Sheffield WednesdayBarnsley 1-2 Sheffield Wednesday | Sky Bet Championship |
| 41 | Fri 2 Apr 2021 | 71-1 v ReadingBarnsley 1-1 Reading | Sky Bet Championship |
| 42 | Mon 5 Apr 2021 | 72-1 v Luton TownLuton Town 1-2 Barnsley | Sky Bet Championship |
| 43 | Sat 10 Apr 2021 | 72-0 v MiddlesbroughBarnsley 2-0 Middlesbrough | Sky Bet Championship |
| 44 | Sun 18 Apr 2021 | 70-2 v Coventry CityCoventry City 2-0 Barnsley | Sky Bet Championship |
| 45 | Wed 21 Apr 2021 | 71-0 v Huddersfield TownHuddersfield Town 0-1 Barnsley | Sky Bet Championship |
| 46 | Sat 24 Apr 2021 | 71-0 v Rotherham UnitedBarnsley 1-0 Rotherham United | Sky Bet Championship |
| 47 | Sat 1 May 2021 | 70-2 v Preston North EndPreston North End 2-0 Barnsley | Sky Bet Championship |
| 48 | Sat 8 May 2021 | 72-2 v Norwich CityBarnsley 2-2 Norwich City | Sky Bet Championship |
| 49 | Mon 17 May 2021 | 70-1 v Swansea CityBarnsley 0-1 Swansea City | Sky Bet Championship Play-off |
| 50 | Sat 22 May 2021 | 71-1 v Swansea CitySwansea City 1-1 Barnsley | Sky Bet Championship Play-off |
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