| F/T | Tonbridge Angels 0-5 Abingdon United - Full-time |
| F/T | Tooting Bec 2-1 Oxford City - Full-time |
| F/T | Solihull Moors 1-1 Grimsby Town - Full-time |
| F/T | Hammersmith 3-2 Wycombe Wanderers - Full-time |
| F/T | Chelmsford City 0-1 Harpenden Town - Full-time |
| F/T | Bowers & Pitsea 3-5 Barking - Full-time |
| F/T | Actonians LFC 7-0 Ascot United - Full-time |
| F/T | Chatham Town 2-1 Farnham Town - Full-time |
| F/T | Yeovil United 1-3 Portishead Town - Full-time |
| F/T | Yate Town 2-0 Bishops Cleeve - Full-time |
| F/T | Wythenshawe 1-2 Thornaby - Full-time |
| F/T | Worthing 1-2 Brentford - Full-time |
| F/T | Worcester City 1-2 Port Vale - Full-time |
| F/T | Wirral Phoenix 0-1 South Shields - Full-time |
| F/T | Wimborne Town 0-4 Moneyfields - Full-time |
| F/T | Walsall 0-6 Redditch Borough - Full-time |
| F/T | Wallsend 0-2 Wigan Athletic - Full-time |
| F/T | Tranmere Rovers 1-5 Bolton Wanderers - Full-time |
| F/T | Stockton Town 1-12 Leeds - Full-time |
| F/T | Stevenage 0-2 Wroxham - Full-time |
| F/T | St Agnes 1-0 Varsity - Full-time |
| F/T | Sporting Khalsa 8-0 Oakham United - Full-time |
| F/T | Sheffield 0-7 Sutton Coldfield Town - Full-time |
| F/T | Saltdean United 3-0 Camden Town - Full-time |
| F/T | Royston Town 3-2 Chesham United - Full-time |
| F/T | Rotherham United 4-0 Spennymoor Town - Full-time |
| F/T | Preston North End 7-0 North Ferriby - Full-time |
| F/T | Nottingham Trent 5-1 Stamford - Full-time |
| F/T | Norton & Stockton 3-1 Salford City - Full-time |
| F/T | Northampton Town 7-0 Sutton United - Full-time |
| F/T | Milton Keynes Dons 5-0 Colchester United - Full-time |
| F/T | Mangotsfield United 2-4 Winchester City Flyers - Full-time |
| F/T | Luton Town 2-1 AFC Sudbury - Full-time |
| F/T | London Bees 0-1 Dartford - Full-time |
| F/T | Leeds Modernians 3-0 Alnwick Town - Full-time |
| F/T | Leafield Athletic 4-1 Shrewsbury Town - Full-time |
| F/T | Kirby Muxloe 0-8 Notts County - Full-time |
| F/T | Islington Borough 0-7 Maidenhead United - Full-time |
| F/T | Ilminster Town 1-3 Feniton - Full-time |
| F/T | Hitchin Belles 0-4 Cambridge United - Full-time |
| F/T | Hednesford Town 0-0 Nantwich Town - Full-time |
| F/T | Harrogate Town 2-1 Altofts - Full-time |
| F/T | Halifax 4-0 York City - Full-time |
| F/T | Gwalia United 6-0 AFC Stoneham - Full-time |
| F/T | AFC Fylde 3-2 Blackburn Rovers - Full-time |
| F/T | Enfield Town 6-0 Billericay Town - Full-time |
| F/T | Dussindale & Hellesdon 3-1 Milton Keynes Irish - Full-time |
| F/T | Dursley Town 0-3 Andover New Street - Full-time |
| F/T | Dulwich Hamlet 1-0 Newhaven - Full-time |
| F/T | Doncaster Rovers Belles 1-1 Workington Town - Workington Town win 4-3 on penalties |
| F/T | Denham United 2-4 Millwall Lionesses - Full-time |
| F/T | Darwen 2-4 Durham Cestria - Full-time |
| F/T | Crewe Alexandra LFC 7-4 Droitwich Spa - Full-time |
| F/T | Coventry Sphinx 2-3 SJR Worksop - Full-time |
| F/T | Chesterfield 10-0 Thurmaston DPC - Full-time |
| F/T | Chester-le-Street Town 3-1 St Helens - Full-time |
| F/T | Bromley 1-0 Reading - Full-time |
| F/T | Bristol Rovers 6-1 Corsham Town - Full-time |
| F/T | Bridgwater United 10-0 Royal Wootton Bassett - Full-time |
| F/T | Bradford City 0-8 Stockport County - Full-time |
| F/T | Bognor Regis Town 0-4 Queens Park Rangers - Full-time |
| F/T | Blyth Spartans 0-9 Chorley - Full-time |
| F/T | Basford United 0-6 Kidderminster Harriers - Full-time |
| F/T | Barnsley 1-2 Mancunian Unity - Full-time |
| F/T | Alvechurch 1-1 Mansfield Town - Full-time |
| F/T | AFC Portchester 4-0 Bournemouth Sports - Full-time |
| F/T | Keynsham Town 2-1 Torquay United - Full-time |
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